What role does hydrogen sulfide play in coronary vascular function? That’s just what lead author Lakshmi Santhanam and colleagues set out to uncover in the work by Kuo et al, which we discuss in this podcast. Associate Editor Nancy Kanagy (University of New Mexico School of Medicine) interviews Santhanam (Johns Hopkins University) and content expert Christopher Kevil (Louisiana State University) about the role and source of hydrogen sulfide in coronary arteries and the discovery that the endothelial enzyme 3-mercaptypyruvate sulfertransferase (MPST) is the primary regulator. Does MPST operate in other vascular beds? Are the fluorescence indicators used by Santhanam and co-authors a reliable tool to detect real-time measurements of hydrogen sulfide production? Listen and find out.